bio rad western blot protein concentration

#BIO RAD WESTERN BLOT PROTEIN CONCENTRATION PLUS#

Introducing the Western Blot Learning Center.The 2-D Doctor is a self-help guide developed by Bio-Rad that enables you to identify and troubleshoot your 2-D gel issues. Troubleshooting 2-D Electrophoresis Gels with 2-D Doctor.Our self-help troubleshooting guide covers solutions to many common and not-so-common western blotting issues and helps your blots look their best. To optimize sample loading, see Determining the Appropriate Sample Load for Western Blots Protocol. Possible causes: Solutions: Sample overloading. Western Blot Doctor Troubleshooting Guide If using a digital imager, you see regions highlighted as saturated.

#BIO RAD WESTERN BLOT PROTEIN CONCENTRATION PLUS#

This guide describes the experimental methods and tools used in 2-D electrophoresis and proteomics research.ĭetails on blotting technology, available products, and methods, plus tips, techniques, and troubleshooting.Ī guide to polyacrylamide gel electrophoresis and protein detection, including theory, product selection, protocols, and more. 2-D Electrophoresis Workflow How-To Guide.Loading buffers for protein or nucleic acid samples, detergents, ampholytes, and gel-casting buffers.įilter by your laboratory set-up and reagents to get a custom western blotting protocol that best fits your needs. Visualize and quantify protein bands including Coomassie stains, fluorescent stains, silver stains, reversible negative stains, and total protein blot stains. Molecular weight standards for applications including SDS-PAGE, western blotting, 2-D PAGE, and isoelectric focusing (IEF). Protein Ladders and Standards (Markers).In-Cell Western Assays are high-throughput quantitative and qualitative immunofluorescence assays that utilizes Bio-Rad's ChemiDoc MP Imaging System and Image Lab Software.ĭetect, image and quantify multiplex fluorescence, chemiluminescence, stain-free and colorimetric blots and gels. Western blotting products include protein transfer systems, membranes, filter paper, blotting buffers, and detection kitsīio-Rad's fluorescent western blotting workflow is a seamless integration of products designed to work together to offer guaranteed results. Our electrophoresis chambers enable rapid, high-resolution protein separation on precast or handcast gels over a variety of different gel sizes.Ĭhoose SDS-PAGE and native-PAGE gels, convert to TGX Precast Gels, or choose specialized gel chemistries including stain-freeįrom basic to high-voltage capacity, our power supplies provide simple programming in a compact, stackable case. Protein Electrophoresis Equipment and Consumables

Bio rad western blot protein concentration